胚胎接种和1日龄肌肉注射鸡传染性贫血病毒 SDLY08株的致病性比较

doi:10.11843/j.issn.0366-6964.2015.03.011

Abstract

Abstract:

This experiment was conducted to compare the pathogenicity，immune suppression，and the horizontal transmission capacity between intramuscular inoculation in 1-day-old chicks and intravenous inoculation in embryos with chicken anemia virus (CAV) strain SDLY08.SPF chickens were divided into four groups：chickens from embryos inoculated intravenously with CAV at 10 days of incubation，1 day-old SPF chicken inoculated with CAV intramuscularly，contact infection group and the control group.The Ratios of thymus and the Bursa to body weigh，indexes of hematology，and CAV antibody levels were examined at 14 and 42 days of age.All groups were immunized with inactivated vaccines to avian influenza virus (AIV-H9N2) and Newcastle disease virus (NDV) at 2 weeks of age，and antibody titers to AIV-H9N2 and NDV were tested at 28，35 and 42 days of age.Results indicated that both CAV-inoculated groups showed growth retardation，severe atrophy of thymus，significant decrease of HI antibody titers to NDV and H9N2，lower levels of red blood cells，white blood cells and the haematocrit (P<0.05) when compared to the control group，but there was no significant difference in these parameters between chicken groups inoculated with different routes (P<0.05).However，chicken group with embryo inoculation demonstrated some mortality (4/25) by 28 days of age and there was no mortality (0/25) in the group inoculated muscularly.Contact infection also induced growth retardation，immunosuppression and anemia，but not as strong as direct inoculation with CAV (P<0.05).At 42 days of age，all chickens inoculated muscularly at 1 day or in embryos developed antibodies positive to CAV，and 95% of in contact infected birds were also antibody positive to CAV， indicating its effective horizontal transmission.

CLC Number:

S852.659.2

CHEN Wen-qing，ZHANG Zhen-jie，ZHAO Peng，DONG Xuan，ZHANG Fu-shou，CUI He，LI Si-fei，CUI Zhi-zhong. The Comparison of Pathogenicity between Intramuscular Inoculation in 1-day-old Chicks and Intravenous Inoculation in Embryos with CAV SDLY08 Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(3): 424-429.

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The Comparison of Pathogenicity between Intramuscular Inoculation in 1-day-old Chicks and Intravenous Inoculation in Embryos with CAV SDLY08 Strain

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